| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | BARNES, Kya | FR | Timberland High School | NT | Unverified: |
| 4 | SWEETEN, Addison | FR | Francis Howell High School | 57.39 | Verified |
| 5 | PAINTER, Sam | FR | Francis Howell Central High Scho | 1:01.54 | Verified |
| 6 | JENSEN, Olivia | FR | Washington High School | NT | Unverified: |