| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | LEWIS, Kalea | FR | Troy Buchanan High School | NT | Unverified: |
| 4 | AYERS, Brynley | FR | Francis Howell High School | NT | Unverified: |
| 5 | MATLOCK, Trinity | FR | Timberland High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | JAENKE, Whitney | FR | Francis Howell High School | NT | Unverified: |
| 3 | BOMMARITO, Camila | FR | Francis Howell High School | NT | Unverified: |
| 4 | CLARK, Miranda | FR | Francis Howell Central High Scho | 1:17.34 | Verified |
| 5 | WILLIAMS, Kristian | FR | Francis Howell Central High Scho | 1:26.34 | Verified |
| 6 | PETERS, Reagan | FR | Troy Buchanan High School | NT | Unverified: |
| 7 | MULLEN, Erika | FR | Timberland High School | NT | Unverified: |