| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Troy Buchanan High School | A | Dowell | Humphrey | Hastings | Vollmer | NT | Unverified: |
| 3 | Washington High School | A | Bush | Henderson | Stegen | Smith | 53.00 | Unverified: |
| 4 | Francis Howell High School | A | 44.77 | Verified | ||||
| 5 | Christian Brothers College High | A | Rivers | Jones | McClellan | Marshall | 46.94 | Unverified: SLUH JV/C #1 |
| 6 | Timberland High School | A | Lewis | Engemann | Neubauer | Cerrano | NT | Unverified: |
| 7 | Francis Howell Central High Scho | A | Bell | Gil Farias | Thornton | Payton | DQ | Unverified: |