| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Timberland High School | A | Sanders | Brown | Prewitt | Barnes | DQ | Unverified: |
| 4 | Francis Howell High School | A | Adelsberger | Barr | Spirz | Lewis | 57.00 | Unverified: |
| 5 | Washington High School | A | Lonsberry | McGee | Mitchell | Jensen | 1:00.00 | Unverified: |