| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Francis Howell North High School | A | Ingracia | Gleason | Abusoud | Scott | 1:42.03 | Verified |
| 4 | Ft. Zumwalt East High School | A | Booker | Terrell | Jones | Bailey | 1:40.28 | Verified |
| 5 | St. Charles West High School | A | Durham | Green | Montalvo | Parrish | 1:41.52 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ft. Zumwalt South High School | A | Beck | Butler | Godefroid | Pilger | 1:40.13 | Verified |
| 2 | Francis Howell Central High Scho | A | Bell | Glacken | Payton | Smith | 1:36.18 | Verified |
| 3 | Ft. Zumwalt North High School | A | Futrell | Hamilton | Miller | Henderson | 1:34.22 | Verified |
| 4 | Ft. Zumwalt West High School | A | 1:31.35 | Verified | ||||
| 5 | Troy Buchanan High School | A | Hopmann | Keene | Moore | LaRue | 1:32.65 | Verified |
| 6 | Warrenton High School | A | Dawson | Brosenne | Lombardo | Bristol | 1:35.10 | Verified |
| 7 | Northwest (Cedar Hill) High Scho | A | Grellner | Frazier | Campbell | Huttenlocker | 1:39.06 | Verified |