| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Northwest (Cedar Hill) High Scho | A | Washington | Campbell | Poskin | Porter | 3:51.88 | Verified |
| 2 | Troy Buchanan High School | A | Freeman | Keene | LaRue | Mahler | 3:41.55 | Verified |
| 3 | Ft. Zumwalt South High School | A | Bates | Bass | Dewitt | Walker | 3:35.38 | Verified |
| 4 | Ft. Zumwalt West High School | A | 3:30.65 | Verified | ||||
| 5 | Ft. Zumwalt North High School | A | Friend | Page | Miller | Ostmann | 3:32.25 | Verified |
| 6 | Warrenton High School | A | Claiborne | Kackley | Allen | Bristol | 3:39.53 | Verified |
| 7 | Francis Howell Central High Scho | A | McDevitt | Kinion | Martin | Jones | 3:47.71 | Verified |