| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ft. Zumwalt South High School | A | Harper | Fenton | Ritter | Pawlik | 4:41.48 | Verified |
| 2 | Warrenton High School | A | Marschel | Morris | Rhama | Marschel | 4:26.18 | Verified |
| 3 | St. Charles West High School | A | Jones | Prost | Myers | Zeier | 4:20.45 | Verified |
| 4 | Francis Howell Central High Scho | A | Hantack | McDevitt | Halterman | Belter | 3:59.40 | Verified |
| 5 | Troy Buchanan High School | A | Arndt | Ellison | Haynes | Peters | 4:20.40 | Verified |
| 6 | Ft. Zumwalt North High School | A | Allen | Abdelkarim | Maier | Jones | 4:25.14 | Verified |
| 7 | Northwest (Cedar Hill) High Scho | A | Surratt | Orozco | Griffin | Shelledy | 4:31.48 | Verified |
| 8 | Holt High School | A | Barley | Kiehl | Adams | Turley | 5:03.70 | Verified |