| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | IKEDINACHI, Chetachi | FR | Ft. Zumwalt East High School | NT | |
| 3 | SMITH, Ella | FR | Ft. Zumwalt East High School | 32.79 | Verified |
| 4 | JOHNSON, Arya | FR | Ft. Zumwalt West High School | 32.04 | Verified |
| 5 | WALDEN, Elizabeth | JR | Ft. Zumwalt West High School | 32.34 | Verified |
| 6 | GATEWOOD, Shelby | SR | Ft. Zumwalt North High School | NT | |
| 7 | STARKEY, Lillian | FR | Troy Buchanan High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LABRASH, Lexie | SO | Northwest (Cedar Hill) High Scho | 30.80 | Unverified: Kirkwood Early Bird |
| 2 | SLUSSER, Jaden | FR | Francis Howell North High School | 30.20 | Unverified: |
| 3 | CROCKETT, Sidney | SO | Holt High School | 29.39 | Verified |
| 4 | KIMBLE, Taylah | JR | Ft. Zumwalt North High School | 29.03 | Verified |
| 5 | G'SELL, Adalynn | FR | Northwest (Cedar Hill) High Scho | 29.10 | Unverified: Kirkwood Early Bird |
| 6 | BOXDORFER, Aaliyah | SR | St. Charles West High School | 30.10 | Unverified: |
| 7 | DANIEL, Kiera | SO | Warrenton High School | 30.48 | Verified |
| 8 | KRAPF, Anna | SO | Ft. Zumwalt South High School | 31.93 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | NOEL, Kiana | FR | Ft. Zumwalt North High School | 28.36 | Verified |
| 2 | OVERKAMP, Kylie | JR | Warrenton High School | 28.05 | Verified |
| 3 | MOORE, Alauna | SO | Ft. Zumwalt South High School | 27.65 | Verified |
| 4 | ANTHONY, Tijaria | SO | Francis Howell Central High Scho | 26.67 | Verified |
| 5 | PETERS, Reagan | FR | Troy Buchanan High School | 26.86 | Verified |
| 6 | LOGAN, Cameryn | SO | Francis Howell Central High Scho | 27.93 | Verified |
| 7 | HOELTING, Jillian | FR | Francis Howell North High School | 28.20 | Unverified: |
| 8 | RAYNER, Joy | JR | Holt High School | 28.98 | Verified |