| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | GERBER, Adam | SR | Northwest (Cedar Hill) High Scho | 48.34 | Verified |
| 3 | THORNTON, Don | FR | Francis Howell Central High Scho | 46.54 | Verified |
| 4 | SUTER, Adam | JR | Ft. Zumwalt West High School | 45.44 | Verified |
| 5 | CAMERON, Isaias | FR | Ft. Zumwalt West High School | 45.99 | Verified |
| 6 | POSKIN, Gage | SR | Northwest (Cedar Hill) High Scho | 48.20 | Verified |
| 7 | PAGE, TaDarius | SR | Francis Howell Central High Scho | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SANDERS, Lynden | JR | Ft. Zumwalt North High School | 44.61 | Verified |
| 2 | DOWDY, Gilbren | SR | Francis Howell North High School | 44.13 | Verified |
| 3 | EDWARDS, Harrison | SR | Troy Buchanan High School | 43.43 | Verified |
| 4 | DEWITT, Xander | JR | Ft. Zumwalt South High School | 40.65 | Verified |
| 5 | LARUE, Lucas | JR | Troy Buchanan High School | 42.37 | Verified |
| 6 | ALLEN, Jordan | SR | Francis Howell North High School | 44.07 | Verified |
| 7 | CURRAN, Jack | SO | Holt High School | 44.35 | Verified |
| 8 | JONES, Emanuel | SO | Ft. Zumwalt North High School | NT |