| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | LADEVICH, Kathryn | SR | Ft. Zumwalt West High School | 1:09.02 | Verified |
| 4 | TUDOR, Sara | JR | Holt High School | 57.52 | Verified |
| 5 | CHAUDHARI, Isha | SO | Ft. Zumwalt West High School | 1:00.69 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | REESE, Makayla | JR | Ft. Zumwalt South High School | 55.59 | Verified |
| 3 | HAAS, Lexi | SO | Northwest (Cedar Hill) High Scho | 55.34 | Verified |
| 4 | SOMMER, Paige | FR | Warrenton High School | 54.48 | Verified |
| 5 | PAINTER, Sam | FR | Francis Howell Central High Scho | 54.74 | Verified |
| 6 | OXFORD, Savannah | FR | Ft. Zumwalt East High School | 55.37 | Verified |
| 7 | ZEUSCHEL, Mariah | SO | Holt High School | 57.07 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GETZ, Brynna | SO | Troy Buchanan High School | 53.96 | Verified |
| 2 | HANTACK, Samantha | JR | Francis Howell Central High Scho | 53.82 | Verified |
| 3 | BROWN, Teryn | JR | Troy Buchanan High School | 53.27 | Verified |
| 4 | DOUGLAS, Triniti | SR | Ft. Zumwalt South High School | 50.00 | Verified |
| 5 | ZEIER, Alivia | JR | St. Charles West High School | 52.74 | Verified |
| 6 | GRIFFIN, J'Kyla | SR | Northwest (Cedar Hill) High Scho | 53.62 | Unverified: JV Marquette |
| 7 | DANIEL, Kiera | SO | Warrenton High School | 53.92 | Verified |
| 8 | MAIER, Savannah | JR | Ft. Zumwalt North High School | 54.36 | Verified |