| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | VOLLMAR, Jackson | SO | Francis Howell North High School | 1:02.03 | Unverified: |
| 4 | STROER, Kadin | SO | Warrenton High School | 1:00.69 | Verified |
| 5 | SMITH, Lucas | JR | Northwest (Cedar Hill) High Scho | 1:01.17 | Verified |
| 6 | ANZALDUA CAMPOS, Roy | FR | Ft. Zumwalt South High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PENBERTHY, Micah | FR | Northwest (Cedar Hill) High Scho | 58.34 | Verified |
| 2 | MCGHEE, Jesse | SO | Troy Buchanan High School | 57.24 | Verified |
| 3 | DUBOWSKI, Joseph | SR | Ft. Zumwalt West High School | 56.60 | Verified |
| 4 | HOLLIDAY, Bryson | FR | Holt High School | 55.49 | Verified |
| 5 | DEWITT, Xander | JR | Ft. Zumwalt South High School | 55.86 | Verified |
| 6 | FAJARDO, Jerecho | JR | Francis Howell North High School | 56.78 | Verified |
| 7 | BROSENNE, Gage | JR | Warrenton High School | 58.00 | Verified |
| 8 | FILES, Jeffery | JR | Ft. Zumwalt North High School | 1:00.56 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SMITH, Adam | JR | Ft. Zumwalt West High School | 54.43 | Verified |
| 2 | KINION, Landon | JR | Francis Howell Central High Scho | 53.67 | Verified |
| 3 | MILLER, Brayden | SR | Ft. Zumwalt North High School | 51.13 | Verified |
| 4 | ANDERSON, Tyler | JR | Ft. Zumwalt East High School | 50.78 | Verified |
| 5 | POLITTE, Braedyn | SR | St. Charles West High School | 51.10 | Verified |
| 6 | OSTMANN, Chase | SO | Ft. Zumwalt North High School | 53.01 | Verified |
| 7 | MAHLER, Thomas | JR | Troy Buchanan High School | 54.22 | Verified |
| 8 | MARTIN, Andrew | SO | Francis Howell Central High Scho | 54.58 | Verified |