| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | RAU, Leah | FR | Ft. Zumwalt South High School | NT | Unverified: |
| 4 | LARAMIE, Lillian | FR | Ft. Zumwalt West High School | 1:10.30 | Verified |
| 5 | STETHEM, Wanda | SO | Northwest (Cedar Hill) High Scho | 1:12.68 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | KIEHL, Natalie | FR | Holt High School | 1:09.89 | Verified |
| 3 | GROOMS, Briana | JR | Warrenton High School | 1:08.94 | Verified |
| 4 | BELTER, Sydney | SO | Francis Howell Central High Scho | 1:07.18 | Verified |
| 5 | GARCIA, Rebecca | FR | Ft. Zumwalt West High School | 1:07.78 | Verified |
| 6 | KIMBLE, Taylah | JR | Ft. Zumwalt North High School | 1:09.39 | Verified |
| 7 | BARLEY, Lillian | SR | Holt High School | 1:09.95 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HALTERMAN, Hannah | SO | Francis Howell Central High Scho | 1:06.29 | Verified |
| 2 | LEWIS, Kalea | FR | Troy Buchanan High School | 1:05.76 | Verified |
| 3 | PAWLIK, Bryn | SR | Ft. Zumwalt South High School | 1:03.44 | Verified |
| 4 | MARSCHEL, Morgan | SO | Warrenton High School | 59.99 | Verified |
| 5 | SURRATT, Cami | JR | Northwest (Cedar Hill) High Scho | 1:02.38 | Verified |
| 6 | JONES, Peyton | SO | Ft. Zumwalt North High School | 1:03.92 | Verified |
| 7 | HEAD, Katelyn | FR | Francis Howell North High School | 1:05.78 | Verified |
| 8 | GIETL, Olivia | FR | Francis Howell North High School | 1:06.77 | Verified |