| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Ft. Zumwalt East High School | A | Booker | Terrell | Jones | Bailey | 48.96 | Verified |
| 4 | Ft. Zumwalt South High School | A | Beck | Butler | Godefroid | Pilger | 46.37 | Verified |
| 5 | Northwest (Cedar Hill) High Scho | A | Grellner | Frazier | Brandt | Huttenlocker | 47.68 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Francis Howell Central High Scho | A | Bell | Glacken | Payton | Smith | 45.84 | Verified |
| 2 | Warrenton High School | A | Dawson | Brosenne | Lombardo | Bristol | 45.47 | Verified |
| 3 | Ft. Zumwalt West High School | A | 44.44 | Verified | ||||
| 4 | Troy Buchanan High School | A | Hopmann | Hall | Moore | Jones | 43.55 | Verified |
| 5 | Ft. Zumwalt North High School | A | Jones | Pruitt | Henderson | Futrell | 44.44 | Verified |
| 6 | Francis Howell North High School | A | Golden | Abusoud | Fajardo | Paul | 44.97 | Verified |
| 7 | Holt High School | A | Akano | Adams | Hartweck | Macklin | 45.70 | Verified |
| 8 | St. Charles West High School | A | Buehrle | Durham | Green | Montalvo | 46.25 | Verified |