| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Francis Howell North High School | A | Burris | Walters | Slusser | Gietl | 1:09.10 | Verified |
| 4 | Northwest (Cedar Hill) High Scho | A | 56.14 | Verified | ||||
| 5 | Ft. Zumwalt East High School | A | Carter | Foster | Albers | Ikedinachi | 57.10 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ft. Zumwalt South High School | A | Moller | Reese | Krapf | Moore | 54.73 | Verified |
| 2 | Holt High School | A | Payne | Crockett | Watkins | Rayner | 54.29 | Verified |
| 3 | Ft. Zumwalt North High School | A | Michel | Kimble | Hamilton | Noel | 52.64 | Verified |
| 4 | Troy Buchanan High School | A | Hunolt | Peters | Trower | Ellison | 51.96 | Verified |
| 5 | Francis Howell Central High Scho | A | Logan | Williams | Johnson | Belter | 52.00 | Verified |
| 6 | Warrenton High School | A | Morris | Marschel | Rhama | Overkamp | 52.81 | Verified |
| 7 | St. Charles West High School | A | O'Kelley | Turner | Prost | Myers | 54.66 | Verified |
| 8 | Ft. Zumwalt West High School | A | Evans | Munyaka | Gaddhi | Sreekanth | 55.86 | Verified |