| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ft. Zumwalt South High School | A | Godefroid | Calder | McCray | Milliner | NT | Unverified: |
| 2 | Rockwood Summit High School | A | Amy | Hanse | Jackson | MUJAGIC | 3:42.59 | Unverified: |
| 7 | Ft. Zumwalt East High School | A | Borger | Cravens | Hoette | Nair | NT | Unverified: |