| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BATES, Milo | FR | Ft. Zumwalt South High School | NT | Unverified: |
| 2 | TERRELL, Sean | JR | Ft. Zumwalt East High School | NT | Unverified: |
| 3 | MILLER, Jaylen | JR | Bayless High School | 1:01.75 | Verified |
| 4 | UEBARI, David | SR | Bayless High School | 51.74 | Verified |
| 5 | BASS, Luke | JR | Ft. Zumwalt South High School | 54.76 | Verified |
| 6 | WILEY, Tyrone | SO | Jennings High School | 1:02.08 | Verified |
| 7 | SMITH, Darius | SR | Jennings High School | NT | Unverified: |
| 8 | NORRIS, Ryan | JR | Ft. Zumwalt East High School | NT | Unverified: |