| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ft. Zumwalt South High School | A | Godefroid | Graham | Milliner | Calder | NT | Unverified: |
| 2 | Ft. Zumwalt East High School | A | Kinder | Stefaniak | Searing | Spears | NT | Unverified: |
| 6 | Rockwood Summit High School | A | Amy | Jackson | MUJAGIC | Sander | 47.40 | Unverified: |
| 7 | Bayless High School | A | Saville | Hulsey | Miller | Bertrand | NT | Unverified: |