| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Parkway Central High School | A | Kruger | Brown | Washington | Douthit | NT | Unverified: |
| 4 | Lindbergh High School | A | Earley | Watts | Chavez | Birk | 1:43.20 | Verified |
| 5 | Parkway South High School | A | Eason | Ashman | Bryant | Ingram | 1:44.46 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mehlville High School | A | Clayton | Evans | Guittar | Jones | 1:37.03 | Unverified: |
| 2 | Webster Groves High School | A | Allen | Booth | Bias | Richardson | 1:35.72 | Verified |
| 3 | Ritenour High School | A | McCarty | Mitchell-Williams | Bishop | Watson | NT | Unverified: |
| 4 | Christian Brothers College High | A | Dubose | Van | Tillman | Hendricks | DQ | Unverified: |
| 5 | St. Louis University High School | A | Harris | Oliphant | Moore | Wingo | 1:31.39 | Unverified: |
| 6 | Eureka High School | A | Bennett | HARRELL | UMBARGER | PETERSON | 1:34.70 | Unverified: |
| 7 | Rolla High School | A | Thomas | Stinson | Kearse | Stallworth | 1:37.02 | Verified |