| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Parkway South High School | A | Duncan | Watkins | Morrow | Lawson | 1:44.46 | Verified |
| 4 | Eureka High School | A | Belloli | Hasler | KOHL | Robinson | 1:43.16 | Verified |
| 5 | Lindbergh High School | A | Brown | Engbert | Fauth | Frantti | 1:43.20 | Verified |
| 6 | Parkway Central High School | A | Ruckstaetter | Lodhi | Taylor | Porter | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Rolla High School | A | Plank | Harris | Hamer | Libre | 1:40.00 | Unverified: |
| 2 | Ritenour High School | A | Vaz | Williams | Lucious | Mitchell | 1:38.00 | Unverified: |
| 3 | Christian Brothers College High | A | Jackson | Jones | Rivers | Marshall | 1:36.00 | Unverified: |
| 4 | St. Mary's (St. Louis) High Scho | A | Ford | Booker | Scott-Brewer | Stevenson | 1:33.08 | Verified |
| 5 | Webster Groves High School | A | Abotsi | Weir | Salmon | Robinson | 1:35.72 | Verified |
| 6 | DeSmet Jesuit High School | A | Griffin | Tuffli | Robinson | Houston | 1:36.55 | Verified |
| 7 | St. Louis University High School | A | Kreuter | Bennett | Carson | Chaffee | 1:38.39 | Unverified: |
| 8 | Mehlville High School | A | Randle | Jones | Hannam-Lewis | Cody | 1:41.03 | Unverified: |