| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Webster Groves High School | A | Richardson | Wetzel-Meehan | Buckman | Cole | 8:33.22 | Unverified: Kirkwood EB |
| 2 | Parkway South High School | A | Metcalf | Tatro | Rebholz | Garimella | 8:42.66 | Verified |
| 3 | St. Louis University High School | A | Murray | Fyvie | Evers | Rammacher | 8:46.33 | Verified |
| 4 | Lindbergh High School | A | Wible | Wightman | Sweeney | Liebetreu | 8:59.60 | Verified |
| 5 | DeSmet Jesuit High School | A | Poelker | Chickering | Alu | Shaughnessy | 9:01.39 | Verified |
| 6 | Rolla High School | A | Aguilarleon | Shenefield | Lortz | Pratz | 9:04.87 | Verified |
| 7 | Eureka High School | A | BARTH | MCAULIFFE | Cook | Watkins | 9:15.00 | Unverified: |