| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Parkway South High School | A | Ashman | Schellman | Lawson | Kim | NT | Unverified: |
| 4 | Mehlville High School | A | Castaldi | Mackey | Ordaz | Whelehon | 4:01.80 | Verified |
| 5 | Parkway Central High School | A | Bedoya Garcia | Kruger | Douthit | Brown | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Christian Brothers College High | A | Drummond | Hayden | Terrell | Wertel | NT | Verified |
| 3 | Rolla High School | A | Stinson | Thomas | Lortz | Briggs | 3:45.97 | Verified |
| 4 | St. Louis University High School | A | Mayer | Franks | Jordan | Talleur | 3:28.85 | Verified |
| 5 | Eureka High School | A | PETERSON | Howlett | Brown | Hotop | 3:37.50 | Unverified: |
| 6 | Webster Groves High School | A | McClure | Wilson | Hotze | Thompson | 3:48.27 | Verified |
| 7 | Lindbergh High School | A | Deterding | Wible | Hilton | Wightman | 3:56.60 | Verified |