| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Parkway South High School | A | Essoufi | Manganiello | Zeloski | Schaefferkoetter | NT | Unverified: |
| 4 | Mehlville High School | A | Watson | Storch | Sanders | McClure | 4:01.80 | Verified |
| 5 | Parkway Central High School | A | Friedman | Zimmer | Porter | Porter | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Christian Brothers College High | A | Strong | Floyd | Glasper | Braddock | 3:55.10 | Verified |
| 2 | St. Louis University High School | A | Baur | Bognar | Strohschein | Scott | 3:52.85 | Unverified: |
| 3 | DeSmet Jesuit High School | A | Griffin | Houston | Robinson | Terry | 3:48.75 | Verified |
| 4 | St. Mary's (St. Louis) High Scho | A | Hearse | Scott-Brewer | Eberhart | Stevenson | 3:40.39 | Verified |
| 5 | Webster Groves High School | A | Reed | Preuss | Abotsi | Weathersby | 3:48.27 | Verified |
| 6 | Eureka High School | A | Berry | Hasler | Lenger | Belloli | 3:51.36 | Verified |
| 7 | Rolla High School | A | Walker | Lindsey | Simmons | Hamer | 3:53.00 | Unverified: |
| 8 | Lindbergh High School | A | Tesch | Freund | Fauth | Duchek | 3:56.60 | Verified |