| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CROCKETT, Michael | SO | Parkway Central High School | NT | Unverified: |
| 2 | INGRAM, Brandon | SR | Parkway South High School | NT | Unverified: |
| 3 | MOORE, Sakye | JR | DeSmet Jesuit High School | 25.05 | Verified |
| 4 | BIAS, Clark | JR | Webster Groves High School | 24.69 | Verified |
| 5 | STALLWORTH, Jason | SO | Rolla High School | 24.93 | Verified |
| 6 | STEWART, Thomas | JR | DeSmet Jesuit High School | 26.14 | Verified |
| 7 | WATTS, Terrell | JR | Lindbergh High School | NT | Unverified: |
| 8 | TAYLOR, Trevion | SO | Parkway Central High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JONES, Connor | SR | Mehlville High School | 24.12 | Verified |
| 2 | BISHOP, Shayn | SO | Ritenour High School | 24.00 | Unverified: |
| 3 | EATON, Nicholas | JR | Lindbergh High School | 23.94 | Verified |
| 4 | ALLEN, Jakahri | JR | Webster Groves High School | 23.86 | Unverified: Ladue Rams Mini Meet |
| 5 | ASHMAN, Roman | JR | Parkway South High School | 23.91 | Verified |
| 6 | ANDERSON, KIELYN | SR | Eureka High School | 23.95 | Verified |
| 7 | HENDRICKS, Chase | SR | St. Mary's (St. Louis) High Scho | 24.02 | Verified |
| 8 | WASHINGTON, Farand | SR | St. Mary's (St. Louis) High Scho | 24.67 | Unverified: Marianist Meet |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HARRELL, MARVELL | SR | Eureka High School | 23.80 | Verified |
| 2 | EVANS, William | SR | Mehlville High School | 23.60 | Unverified: Oakville Practice meet |
| 3 | DUBOSE, Doreon | JR | Christian Brothers College High | 23.31 | Verified |
| 4 | HENDRICKS, Amar'e | SO | Christian Brothers College High | 22.28 | Verified |
| 5 | OLIPHANT, Jacobi | JR | St. Louis University High School | 22.57 | Verified |
| 6 | HARRIS, Cornell | JR | Ritenour High School | 23.42 | Verified |
| 7 | KEARSE, Kennedy | SR | Rolla High School | 23.71 | Verified |
| 8 | BAILEY, Kameron | SR | St. Louis University High School | 23.83 | Verified |