| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | WERTEL, Dylan | SR | Christian Brothers College High | NT | Unverified: |
| 4 | MRAZ, Jackson | JR | Parkway Central High School | 46.49 | Verified |
| 5 | MCCLURE, Jordan | JR | Webster Groves High School | 47.19 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CAHILL, James | JR | Rolla High School | 45.97 | Verified |
| 2 | THOMAS, Saraun | JR | Ritenour High School | 45.04 | Verified |
| 3 | HILTON, Wyatt | SO | Lindbergh High School | 44.35 | Verified |
| 4 | ROBINSON, Tyson | JR | Ritenour High School | 43.74 | Verified |
| 5 | GUYTON, Joshua | SO | St. Mary's (St. Louis) High Scho | 44.30 | Verified |
| 6 | MAYER, Steffan | SR | St. Louis University High School | 44.94 | Verified |
| 7 | MOORE, Matthew | SO | St. Louis University High School | 45.74 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KRUGER, Benjamin | JR | Parkway Central High School | 43.03 | Verified |
| 2 | CLAYTON, Kirion | SR | Mehlville High School | 42.49 | Verified |
| 3 | UMBARGER, RYAN | SR | Eureka High School | 42.27 | Verified |
| 4 | BROWN, Allen | JR | Eureka High School | 40.64 | Verified |
| 5 | VALENTINE, Antonio | SR | St. Mary's (St. Louis) High Scho | 41.92 | Verified |
| 6 | KISSINGER, Hayden | JR | Rolla High School | 42.48 | Verified |
| 7 | DETERDING, Charles | SR | Lindbergh High School | 43.00 | Verified |
| 8 | SPAETE JR, Christopher | SR | Christian Brothers College High | 43.40 | Unverified: |