| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ALLEN, Eric | SR | Parkway Central High School | 1:24.46 | Verified |
| 3 | HARPER, Tyler | FR | Parkway Central High School | NT | Unverified: |
| 4 | BOOKER, Gabe | SR | St. Mary's (St. Louis) High Scho | NT | Unverified: |
| 5 | SARTORIUS, Aden | SO | Lindbergh High School | NT | Unverified: |
| 6 | JEANMOUGIN, Evan | FR | Lindbergh High School | NT | Unverified: |
| 7 | FAUTH, Sylvio | SO | Lindbergh High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HENDRIX, JaMont'e | FR | Christian Brothers College High | 1:05.00 | Unverified: |
| 2 | CANADY, Frederick | SO | Mehlville High School | 1:04.25 | Unverified: Cape Invitational |
| 3 | ESSOUFI, Ahmed | SO | Parkway South High School | 1:02.58 | Verified |
| 4 | HERRMAN, Joel | FR | Webster Groves High School | 1:01.95 | Verified |
| 5 | PORTER, Caleb | FR | Parkway Central High School | 1:02.02 | Verified |
| 6 | HUNT, Kyle | SO | Eureka High School | 1:02.90 | Unverified: |
| 7 | LEZIEANYA, Chinonso | FR | St. Louis University High School | 1:04.27 | Unverified: |
| 8 | KEEL, Kobe | FR | Christian Brothers College High | 1:07.00 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ABOTSI, Aden | FR | Webster Groves High School | 1:01.22 | Unverified: Kirkwood EB |
| 2 | MANGANIELLO, Robert | SR | Parkway South High School | 1:00.49 | Verified |
| 3 | REED, Jalen | FR | Webster Groves High School | 1:00.19 | Verified |
| 4 | SIMMONS, Conner | SO | Rolla High School | 59.12 | Verified |
| 5 | MCCLURE, Jeremiah | SO | Mehlville High School | 59.31 | Verified |
| 6 | ZELOSKI, Tanner | SR | Parkway South High School | 1:00.23 | Verified |
| 7 | PENNINGTON, JACK | SO | Eureka High School | 1:01.00 | Unverified: |
| 8 | JOHNSON, WESLEY | SO | Eureka High School | 1:01.92 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROBINSON, Brandon | SO | DeSmet Jesuit High School | 58.46 | Unverified: |
| 2 | LINDSEY, Trent | SO | Rolla High School | 58.21 | Verified |
| 3 | MCCLELLAN, Nick | FR | Christian Brothers College High | 56.80 | Unverified: |
| 4 | BRIGGS, Ezekial (Blaze) | SR | Rolla High School | 53.12 | Verified |
| 5 | BAUR, Jeffrey | SR | St. Louis University High School | 56.24 | Verified |
| 6 | BOGNAR, Ethan | SO | St. Louis University High School | 57.23 | Verified |
| 7 | TUFFLI, Andrew | SO | DeSmet Jesuit High School | 58.46 | Verified |
| 8 | WATSON, Alexander | SO | Mehlville High School | 59.09 | Verified |