| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Webster Groves High School | A | Kaanagbara | Logan | Bias | Booth | NT | Verified |
| 4 | DeSmet Jesuit High School | A | Pool | King | Stewart | Moore | NT | Verified |
| 5 | Rolla High School | A | Haas | Mertens | Kearse | Stallworth | 46.06 | Verified |
| 6 | Lindbergh High School | A | Earley | Watts | Goss | Eaton | 48.84 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Parkway South High School | A | Eason | Ashman | Bryant | Ingram | 44.94 | Verified |
| 2 | Mehlville High School | A | Clayton | Guittar | Jones | Evans | 44.85 | Unverified: |
| 3 | Ritenour High School | A | Smith | McCarty | Harris | Robinson | 44.00 | Unverified: |
| 4 | Christian Brothers College High | A | Van | Hendricks | McClellan | Love | 43.22 | Unverified: |
| 5 | St. Louis University High School | A | Harris | Oliphant | Moore | Wingo | DQ | Unverified: |
| 6 | Eureka High School | A | ANDERSON | UMBARGER | Bennett | HARRELL | 44.60 | Unverified: |
| 7 | St. Mary's (St. Louis) High Scho | A | Strong | Washington | Hendricks | Roberts | 44.86 | Verified |
| 8 | Parkway Central High School | A | Taylor | Banks | Crockett | Douthit | DQ | Verified |