| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Rolla High School | A | Plank | Harris | Price | Libre | DQ | Unverified: |
| 4 | Eureka High School | A | Belloli | Davis | Hasler | Robinson | 47.72 | Unverified: |
| 5 | St. Louis University High School | A | Kreuter | Chaffee | Carson | Bennett | 47.79 | Unverified: |
| 6 | Lindbergh High School | A | Brown | Frantti | Freels | Duchek | DQ | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Christian Brothers College High | A | Pauley | Stimage | Sargent | Washington | 47.57 | Verified |
| 2 | Webster Groves High School | A | Abotsi | Weir | Salmon | Robinson | 46.16 | Verified |
| 3 | Parkway Central High School | A | Ruckstaetter | Lodhi | Taylor | Porter | 45.95 | Verified |
| 4 | St. Mary's (St. Louis) High Scho | A | Hearse | Scott-Brewer | Eberhart | Stevenson | 44.86 | Verified |
| 5 | Parkway South High School | A | Houston | Foster | Lawson | Lyons | NT | Verified |
| 6 | DeSmet Jesuit High School | A | Byerley | Terry | McCaster | Griffin | NT | Verified |
| 7 | Ritenour High School | A | Barnett | Williams | Jackson | Miller | DQ | Unverified: |
| 8 | Mehlville High School | A | Cody | Moehlenbrock | Shelton | Traore | 47.61 | Unverified: |