| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Mehlville High School | C | Adams | Cohen-Edwards | Alhalabi | Traore | NT |
| 4 | Kirkwood High School | C | Motti | Carnell | Kuhn | Laciny | 2:15.80 |
| 5 | Lafayette (Wildwood) High School | C | Saddler | DQ |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Seckman High School | A | Cline | Mueller | Cardinale | Hunt | 2:15.00 |
| 2 | Parkway North High School | A | Harper | Yeye | Whitley | Spears | NT |
| 3 | Liberty (Wentzville) High School | B | OBrien | Shuls | Weber | Barnes | DQ |
| 4 | Webster Groves High School | C | Blum | Gastreich | Nguyen | Lee | 2:06.00 |
| 5 | Cor Jesu Academy | B | 2:06.03 | ||||
| 6 | Lafayette (Wildwood) High School | B | Mohlman | 2:10.00 | |||
| 7 | Mehlville High School | B | Nava | Wilder | Burnett | McGinnist | 2:12.38 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Webster Groves High School | B | Cannon | Riefle | McCoy | Robinson | 2:04.00 |
| 2 | Lindbergh High School | A | Kobe | Pyle | Vogler | Long | 2:03.07 |
| 3 | Lindbergh High School | B | Lord | Cruzen | Mujkanovic | Causevic | 2:03.07 |
| 4 | Kirkwood High School | B | Barry | Barry | Goodwin | Savage | 2:02.60 |
| 5 | Cor Jesu Academy | A | Gremaud | Detweiler | Mathews | Steiner | 2:02.78 |
| 6 | Lindbergh High School | C | Fleming | Dooling | Godfrey | Groupp | 2:03.07 |
| 7 | Mehlville High School | A | Shores | Tran | Wheeler | Ramos | 2:03.38 |
| 8 | Rockwood Summit High School | C | 2:05.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Liberty (Wentzville) High School | A | Jackson | Vazquez | Kemp | Weishaar | 2:01.81 |
| 2 | Parkway West High School | A | Josef | Phillips | Sanders | Ivanova | 2:00.00 |
| 3 | Eureka High School | B | 1:59.50 | ||||
| 4 | Kirkwood High School | A | Wright | MacLellan | Howell | Pride | 1:57.80 |
| 5 | Lafayette (Wildwood) High School | A | Alvarez | 1:58.00 | |||
| 6 | Eureka High School | C | 2:00.00 | ||||
| 7 | Rockwood Summit High School | B | 2:00.00 | ||||
| 8 | Webster Groves High School | A | Lawless | Oesterlei | Fischer | Knox | 2:01.95 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Rockwood Summit High School | A | 1:56.70 | ||||
| 2 | McCluer North High School | A | Davis | Jefferson | Clayton | Hart | 1:54.19 |
| 3 | Rolla High School | A | Alamaldein | Chance | Brooks | Toney | 1:53.84 |
| 4 | Parkway South High School | A | Hilgendorf | Jones | McHale | Rosewell | 1:52.42 |
| 5 | Ladue Horton Watkins High School | A | DQ | ||||
| 6 | McCluer North High School | B | Davis | Moore | Lamb | Eubanks | NT |
| 7 | Liberty (Wentzville) High School | C | Queen | Wokurka | Hoselton | Sikes | 1:54.81 |
| 8 | Eureka High School | A | 1:57.00 |