| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Ladue Horton Watkins High School | A | NT | ||||
| 3 | Parkway South High School | B | Schjenken | Zheng | Polach | Koenig | 4:23.00 |
| 4 | Christian Brothers College High | B | Gillespie | Cobb | Strong | Braddock | 4:03.10 |
| 5 | Christian Brothers College High | C | Hughes | Jackson | Keel | Floyd | NT |
| 6 | Parkway South High School | C | Schjenken | Ullom | Drinkard | Essoufi | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Webster Groves High School | C | Wilson | Abotsi | Hahn | Hotze | 4:01.22 |
| 2 | Liberty (Wentzville) High School | B | Struttmann | Shuster | Hughes | Bill | 3:59.64 |
| 3 | Mehlville High School | A | Ramos | Nasufovic | McClure | Becerra | 3:58.11 |
| 4 | Kirkwood High School | B | Araneta | Burns | Johnson | Scheidt | 3:53.00 |
| 5 | Webster Groves High School | B | Ahrens | Parikh | O'Brien | Lang | 3:56.63 |
| 6 | Mehlville High School | B | Whelehon | Sanders | Murphy | Arnaiz | 3:59.11 |
| 7 | Parkway West High School | B | Li | Larson | Patten | Collop | 4:00.00 |
| 8 | Mehlville High School | C | Shaw | Evans | Jackson | Sasic | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Liberty (Wentzville) High School | A | Coyle | Orf | Rogers | Foerstel | 3:52.64 |
| 2 | Christian Brothers College High | A | Rodgers | McClellan | Marshall | Leicht | 3:50.10 |
| 3 | St. Louis University High School | A | Jordan | Franks | Rammacher | Bognar | NT |
| 4 | Eureka High School | B | 3:45.24 | ||||
| 5 | Webster Groves High School | A | Doyle | Wetzel-Meehan | Abotsi | Wright | 3:48.27 |
| 6 | Parkway South High School | A | Bryant | Winzen | LeCave | Roethlisberger | NT |
| 7 | Lafayette (Wildwood) High School | B | 3:51.96 | ||||
| 8 | Lafayette (Wildwood) High School | A | SNYDER | BERGER | alsop | Vuskamalla | 3:52.96 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Kirkwood High School | A | Robinson | McKee | Richman | Earl | 3:43.40 |
| 2 | Parkway North High School | A | Tate | Powell | Griffin | Moore | NT |
| 3 | Eureka High School | A | PRINCE | Preston | PENNINGTON | JOHNSON | 3:41.53 |
| 4 | Rolla High School | A | Williams | Lindsey | Elliott | Johnson | 3:36.70 |
| 5 | Parkway West High School | C | Spangler | Clancy | Barry | DeBauch | NT |
| 6 | Liberty (Wentzville) High School | C | Willnerd | Leech | Velleca | Carpenter | 3:42.64 |
| 7 | Kirkwood High School | C | Webb | Wollaeger | Washington | Lindsay | 3:43.40 |
| 8 | Parkway West High School | A | Gaddy | Smith | Strauss | Piel | 3:45.00 |