| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | HOLDER, Bradley | JR | Webster Groves High School | 2:25.46 |
| 2 | SZACHIESKI, Logan | FR | Christian Brothers College High | 2:25.60 |
| 3 | LINDSEY, Trent | SO | Rolla High School | 2:27.49 |
| 4 | CLANCY, Frank | FR | Parkway West High School | 2:28.38 |
| 5 | SCHJENKEN, Katriel | SO | Parkway South High School | 2:29.60 |
| 6 | LIPP, Austin | SO | Seckman High School | 2:29.82 |
| 7 | JACKNEWITZ, Andrew | FR | DeSmet Jesuit High School | 2:29.90 |
| 8 | FLOYD, Kyle | FR | Christian Brothers College High | 2:32.25 |
| 9 | DAVIDSON, Guy | FR | Christian Brothers College High | 2:32.40 |
| 10 | RODRIGUEZ, Carlos | JR | Rolla High School | 2:34.12 |
| 11 | DIEKMANN, KENNETH | SO | Eureka High School | 2:37.16 |
| 12 | DEBAUCH, Dane | FR | Parkway West High School | 2:47.09 |
| 13 | LUGO ESCOBEDO, Enrique | FR | Lafayette (Wildwood) High School | 2:48.60 |
| 14 | THOMAS, Camden | JR | Parkway North High School | 2:49.11 |
| 15 | JENNING, Elijah | FR | Seckman High School | 3:01.76 |
| 16 | SCHULTZ, Cayden | FR | Liberty (Wentzville) High School | 3:04.28 |
| 17 | CURTIS, Charles | FR | Webster Groves High School | NT |
| 18 | SHAW, Oliver | FR | Mehlville High School | NT |
| 19 | GOWEN, Sam | FR | Webster Groves High School | NT |
| 20 | YE, Luke | FR | Ladue Horton Watkins High School | NT |
| 21 | SEHY, Jack | FR | Ladue Horton Watkins High School | NT |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | FERKEL, Peyton | SO | Kirkwood High School | 2:09.58 |
| 2 | SNYDER, LUKE | JR | Lafayette (Wildwood) High School | 2:10.10 |
| 3 | PURSCHKE, Nicholas | SR | St. Louis University High School | 2:10.95 |
| 4 | SCARGALL, Mason | JR | St. Louis University High School | 2:12.00 |
| 5 | ALSOP, Sagan | JR | Lafayette (Wildwood) High School | 2:12.64 |
| 6 | RICHMAN, George | SO | Kirkwood High School | 2:13.08 |
| 7 | LECAVE, Charlie | JR | Parkway South High School | 2:14.40 |
| 8 | WINZEN, Nicholas | SO | Parkway South High School | 2:15.30 |
| 9 | RAMOS, Dominic | SO | Mehlville High School | 2:15.80 |
| 10 | ARNAIZ, Aiden | SO | Mehlville High School | 2:15.86 |
| 11 | SHAUGHNESSY, Jack | SR | DeSmet Jesuit High School | 2:16.10 |
| 12 | ANDERSON, Benjamin | SR | DeSmet Jesuit High School | 2:19.32 |
| 13 | PRINCE, FREDRICK | SO | Eureka High School | 2:19.41 |
| 14 | VOIGT, Nicholas | FR | Parkway North High School | 2:19.93 |
| 15 | JONES, Patrick | SO | St. Louis University High School | 2:20.00 |
| 16 | AGUILAR, Steven | SR | Kirkwood High School | 2:20.65 |
| 17 | FRANCO, Jeremy | SO | Liberty (Wentzville) High School | 2:21.15 |
| 18 | GILL, Owen | JR | Parkway North High School | 2:21.17 |
| 19 | SPANGLER, Cameron | FR | Parkway West High School | 2:21.94 |
| 20 | WATKINS, Ryan | JR | Eureka High School | 2:23.10 |
| 21 | JUDGE, Trevor | SO | Liberty (Wentzville) High School | 2:23.44 |
| 22 | BUCK, Zachary | SO | Seckman High School | 2:24.92 |