| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | RAIBLEY, Audrey | FR | Ladue Horton Watkins High School | 2:59.42 |
| 2 | POHLSANDER, Alexandria | FR | Rolla High School | 2:59.53 |
| 3 | BAUMGARTNER, Amelia | FR | Kirkwood High School | 2:59.56 |
| 4 | TURNBOUGH, ALIVIA | SO | Eureka High School | 2:59.96 |
| 5 | MCSPADDEN, Mirra | FR | Liberty (Wentzville) High School | 3:01.87 |
| 6 | MEIRINK, Anna | JR | Cor Jesu Academy | 3:03.12 |
| 7 | DAVIS, Kara | FR | Seckman High School | 3:05.81 |
| 8 | PATNEAU, Emma | SO | Webster Groves High School | 3:11.65 |
| 9 | CURTIS, Madyson | FR | Rolla High School | 3:14.00 |
| 10 | ROBERTS, Lindsay | SO | Mehlville High School | 3:14.47 |
| 11 | MARTIN, Brynna | FR | Liberty (Wentzville) High School | 3:14.62 |
| 12 | THOMPSON, Kaylee | FR | Parkway South High School | 3:17.09 |
| 13 | FLICK, Elizabeth | JR | McCluer North High School | 3:21.92 |
| 14 | HOGAN, Margaret | SO | Cor Jesu Academy | 3:30.60 |
| 15 | DOW, Kennedy | SO | Parkway South High School | 3:34.20 |
| 16 | OKOLI, Chidinma | FR | McCluer North High School | 3:36.46 |
| 17 | WOODS, Sara | JR | Lafayette (Wildwood) High School | 3:53.91 |
| 18 | GLAVIN, Lexi | SR | Liberty (Wentzville) High School | 3:58.22 |
| 19 | POZZO, Grace | SR | Cor Jesu Academy | NT |
| 20 | MUJIC, Lejla | FR | Mehlville High School | NT |
| 21 | TUMMALA, Deekshanjani | JR | Parkway West High School | NT |
| 22 | BRIANZI, Maya | JR | Ladue Horton Watkins High School | NT |
| 23 | CLAYTON, Cortisha | FR | McCluer North High School | NT |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | GRIFFIN, Karina | SO | Ladue Horton Watkins High School | 2:30.56 |
| 2 | SMITH, Beatrice | FR | Lindbergh High School | 2:39.15 |
| 3 | MCGINNIS, Anna | JR | Kirkwood High School | 2:40.95 |
| 4 | DANTUONO, Lila | SO | Lafayette (Wildwood) High School | 2:42.00 |
| 5 | SOTO, Cristina | SO | Lindbergh High School | 2:43.36 |
| 6 | MURPHY, Sophia | SO | Parkway South High School | 2:44.35 |
| 7 | FAIRBANKS, Rebecca | JR | Webster Groves High School | 2:44.38 |
| 8 | THIER, Alexis | FR | Rockwood Summit High School | 2:44.84 |
| 9 | COLE, Caroline | SR | Rockwood Summit High School | 2:47.65 |
| 10 | HIPP, Olivia | SO | Lindbergh High School | 2:47.74 |
| 11 | MILLER, Allison | SO | Mehlville High School | 2:48.01 |
| 12 | FISH, Gemma | SO | Parkway North High School | 2:48.42 |
| 13 | CUNNINGHAM, Avalon | FR | Eureka High School | 2:49.00 |
| 14 | HUMES, CHLOE | SO | Eureka High School | 2:51.41 |
| 15 | LOETHEN, Grace | SO | Parkway West High School | 2:52.34 |
| 16 | FISCHER, Macey | JR | Webster Groves High School | 2:53.59 |
| 17 | PERSTROPE, Chloe | SO | Rockwood Summit High School | 2:54.24 |
| 18 | MARIC, Emma | SO | Kirkwood High School | 2:54.29 |
| 19 | SKIPTON, Amber | SO | Lafayette (Wildwood) High School | 2:54.74 |
| 20 | STEVENS, Isabella | SO | Seckman High School | 2:55.12 |
| 21 | JANSEN, Eva | SO | Parkway North High School | 2:56.09 |
| 22 | MORTENSEN, Johanne | JR | Parkway West High School | 2:56.84 |
| 23 | UPHOFF, Amelia | FR | Parkway North High School | 2:57.57 |