| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 3 | GREEN, Micaiah | FR | Parkway North High School | NT |
| 4 | TONEY, Addison | FR | Rolla High School | NT |
| 5 | GAVORNIK, Ashlyn | SR | Parkway South High School | NT |
| 6 | BLUE, Olivia | FR | Ladue Horton Watkins High School | NT |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | STARNS, Bridgette | FR | Rolla High School | NT |
| 2 | SANDERS, Hallie | SO | Lindbergh High School | 1:06.00 |
| 3 | FRAZIER, Alexandria | SO | Parkway South High School | 1:01.01 |
| 4 | BOCQUILLON, Rebecca | SO | Lindbergh High School | 1:00.18 |
| 5 | SUMMERS, Cora | FR | Seckman High School | 1:00.41 |
| 6 | MONTROY, Sierra | FR | Lindbergh High School | 1:01.95 |
| 7 | HENRIQUEZ, Stacy | SO | Liberty (Wentzville) High School | 1:07.35 |
| 8 | WHEELER, Julia | SO | Mehlville High School | NT |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | HOWARD, Madelyn | SO | Eureka High School | 1:00.00 |
| 2 | VANDERHOOF, Amara | FR | Eureka High School | 59.79 |
| 3 | NOTTINGHAM, Ella | FR | Eureka High School | 58.83 |
| 4 | CRINNION, Cameron | FR | Seckman High School | 58.64 |
| 5 | MU, Sarah | FR | Lafayette (Wildwood) High School | 58.69 |
| 6 | KOMMU, Lasya | FR | Lafayette (Wildwood) High School | 59.32 |
| 7 | HYMES, Gwen | JR | Cor Jesu Academy | 1:00.00 |
| 8 | JOHNSON, Kaylee | FR | Liberty (Wentzville) High School | 1:00.10 |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | DOHERTY, GRACE | SO | Lafayette (Wildwood) High School | 57.48 |
| 2 | MITCHELL, Alexis | JR | Kirkwood High School | 55.88 |
| 3 | LEIGHT, Emily | JR | Cor Jesu Academy | 55.50 |
| 4 | BANKS, Tristen | JR | Parkway West High School | 53.12 |
| 5 | FORMBY, Sabrina | FR | Seckman High School | 54.09 |
| 6 | MARTZ, Kyrstiana | SO | Kirkwood High School | 55.61 |
| 7 | DAVIS, Sydney | JR | Liberty (Wentzville) High School | 57.15 |
| 8 | MCCLENDON, Nevaeh | FR | Kirkwood High School | 58.06 |