| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Ladue Horton Watkins High School | A | NT | ||||
| 3 | Parkway South High School | A | Houston | Foster | Lyons | Lawson | NT |
| 4 | Eureka High School | B | 52.20 | ||||
| 5 | Lafayette (Wildwood) High School | C | 53.61 | ||||
| 6 | Parkway West High School | A | Barnett | Freeman | Hall | Lipscomb | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Lafayette (Wildwood) High School | B | 50.61 | ||||
| 2 | Liberty (Wentzville) High School | B | Minnix | Mills | Ross | Rey | 49.00 |
| 3 | Webster Groves High School | A | Abotsi | Robinson | Robinson | Salmon | 48.19 |
| 4 | Mehlville High School | C | Sanders | Jackson | Evans | Becerra | 47.86 |
| 5 | Kirkwood High School | C | Barry | Griffin | Loper | Earl | 48.00 |
| 6 | Christian Brothers College High | C | Sommerville | Stimage | Hendrix | Jones | 48.20 |
| 7 | Webster Groves High School | B | Smith | Brewer | Bennett | Weir | 50.19 |
| 8 | Webster Groves High School | C | Wall | Preuss | Sutton | Harris | 51.22 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Eureka High School | A | Davis | MCELROY | Lee | Robinson | 47.65 |
| 2 | Christian Brothers College High | B | Hendrix | Pauley | Cothrine | Williams | 47.10 |
| 3 | Liberty (Wentzville) High School | A | Lawrence | Lively | Hughes | Bill | 47.00 |
| 4 | Parkway North High School | A | Fortenberry | Dickerson | Quarles | Thomas Ali | NT |
| 5 | Kirkwood High School | B | Walter | Johnson | Araneta | Steuby | 47.00 |
| 6 | Seckman High School | A | Nago | Stinchcomb | Johnston | Mraz | 47.00 |
| 7 | Lafayette (Wildwood) High School | A | TINSLEY | Preckel | Tuggles | TUGGLES | 47.61 |
| 8 | Mehlville High School | B | Hannam-Lewis | Jones | McDaniel | Storch | 47.75 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Parkway North High School | B | Griffin | Moore | Tate | Lindsey | 46.50 |
| 2 | Christian Brothers College High | A | Mills | Sargent | Washington | Rivers | 46.20 |
| 3 | Liberty (Wentzville) High School | C | Lamm | Vanhoy | Adams | Burton Wadibia | 46.00 |
| 4 | Rolla High School | A | Gibson | Harris | Hamer | Wassilak | 44.93 |
| 5 | Kirkwood High School | A | McKee | Cauble | Smith | Boswinkel | 45.60 |
| 6 | St. Louis University High School | A | Baur | Pace | Kreuter | Chaffee | 46.13 |
| 7 | DeSmet Jesuit High School | A | Byerley | Houston | Robinson | McCaster | 46.30 |
| 8 | Mehlville High School | A | Shelton | Moehlenbrock | Traore | Guittar | 46.56 |