| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SATTERFIELD, Zafaran | FR | Southern Boone County High Schoo | 5:42.48 | Verified |
| 2 | RIVERA, Anna | SR | Moberly High School | 5:48.44 | Verified |
| 3 | LACKMAN, Clara | JR | Blair Oaks High School | 5:50.94 | Verified |
| 4 | BRENNEKE, Hannah | SR | Blair Oaks High School | 6:06.35 | Verified |
| 5 | RAMSEY, Maggie | FR | Mexico High School | 6:12.00 | Verified |
| 6 | SANTHUFF, Ava | FR | Fulton High School | 6:20.35 | Verified |
| 7 | O'LOUGHLIN, Katherine | FR | Moberly High School | 6:26.40 | Verified |
| 8 | BLEDSOE, TaNaja | SO | Boonville High School | 6:42.23 | Verified |
| 9 | NAVE, Eleanor | FR | Boonville High School | 6:47.71 | Verified |
| 10 | SCHWINKE, Chloe | SO | Fulton High School | 6:52.64 | Verified |
| 11 | BRAGG, Abigail | FR | Christian Fellowship School | 7:29.31 | Verified |