| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Blair Oaks High School | A | Suthoff | Edler | Kesel | Roling | 1:41.27 | Unverified: |
| 2 | Fulton High School | A | Hightower | Tharp | Garrett | Cooper | 1:37.82 | Verified |
| 3 | Mexico High School | A | Shivers | Potter | Charlie) Fisher | Rowan | 1:36.85 | Verified |
| 4 | Moberly High School | A | Gelina | Alexander | Borges | Rasico | 1:32.88 | Verified |
| 5 | Southern Boone County High Schoo | A | Amelunke | Bishop | Lutz | Rehg | 1:36.78 | Verified |
| 6 | Boonville High School | A | Stock | Leonard | Williams | Brackman | 1:37.10 | Verified |
| 7 | Missouri Military Academy | A | Carter | Elford | McBride | Stallone | 1:40.94 | Verified |