| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Blair Oaks High School | A | Fessler | Podorski | Libbert | Griffin | 2:01.90 | Verified |
| 3 | Boonville High School | A | Turner | Thompson | Eichelberger | Imhoff | 1:52.44 | Verified |
| 4 | Moberly High School | A | Dunwoody | Fanning | Hannam | Knox | 1:48.62 | Verified |
| 5 | Fulton High School | A | Weaver | Begemann | Henderson | Hightower | 1:52.06 | Verified |
| 6 | Southern Boone County High Schoo | A | Bukowsky | Germann | Johnson | Patton | 1:52.67 | Verified |