| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Missouri Military Academy | A | Miles | Mugisha | Powell | Salinas | 3:56.11 | Verified |
| 3 | Boonville High School | A | Stock | Williams | Leonard | Brackman | 3:37.61 | Verified |
| 4 | Mexico High School | A | Charlie) Fisher | Grimes | Grubb | Shelton | 3:34.81 | Verified |
| 5 | Southern Boone County High Schoo | A | Marc | Rehg | Burns | Ottinger | 3:35.37 | Verified |
| 6 | Blair Oaks High School | A | Stafford | Boessen | Christian | NT | Verified | |
| 7 | Fulton High School | A | Lozano | Hutchins | Garrett | Dale | 4:00.84 | Verified |