| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Fulton High School | A | Connor | Begemann | Phillippe | Cooper | 4:50.40 | Verified |
| 3 | Boonville High School | A | Turner | Eichelberger | Imhoff | Morris | NT | Verified |
| 4 | Moberly High School | A | Hannam | Knox | Dunwoody | Fanning | 4:14.61 | Verified |
| 5 | Southern Boone County High Schoo | A | Germann | Patton | Volkart | Samuels | NT | Verified |
| 6 | Mexico High School | A | Benjamin | Raines | Seibert | Smythe | 4:38.50 | Verified |