| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | PODORSKI, Samantha | SO | Blair Oaks High School | 32.27 | Verified |
| 4 | FANNING, Addison | FR | Southern Boone County High Schoo | 30.59 | Verified |
| 5 | JOHNSON, Raegan | JR | Southern Boone County High Schoo | 31.56 | Verified |
| 6 | ADAIR, Britney | SR | Christian Fellowship School | 35.19 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROSS, Chloe | SR | Moberly High School | 29.50 | Verified |
| 2 | EICHELBERGER, Alison | JR | Boonville High School | 28.97 | Verified |
| 3 | SMYTHE, Alexis | JR | Mexico High School | 28.75 | Verified |
| 4 | HIGHTOWER, Heaven | FR | Fulton High School | 27.28 | Verified |
| 5 | HESTON, Grace | FR | Christian Fellowship School | 28.08 | Verified |
| 6 | THOMPSON, Cora | SO | Boonville High School | 28.88 | Verified |
| 7 | HENDERSON, Kier | JR | Fulton High School | 29.07 | Verified |
| 8 | CAVINESS, Zoe | JR | Blair Oaks High School | 30.26 | Unverified: |