| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | RIVERA, Anna | SR | Moberly High School | 2:26.95 | Verified |
| 2 | CASEY, Ansley | SR | Blair Oaks High School | 2:28.90 | Verified |
| 3 | SATTERFIELD, Zafaran | FR | Southern Boone County High Schoo | 2:32.74 | Verified |
| 4 | SMOTHERS, Abigail | SR | Moberly High School | 2:41.36 | Verified |
| 5 | BLEDSOE, TaNaja | SO | Boonville High School | 2:45.72 | Verified |
| 6 | SCHWINKE, Chloe | SO | Fulton High School | 2:50.06 | Verified |
| 7 | NAVE, Eleanor | FR | Boonville High School | 2:58.05 | Verified |
| 8 | BENEDICT, Maddie | FR | Blair Oaks High School | 3:06.45 | Unverified: |
| 9 | PATTEN, Greta | FR | Fulton High School | 3:09.82 | Verified |
| 10 | BRAGG, Abigail | FR | Christian Fellowship School | 3:30.12 | Verified |
| 11 | RAMSEY, Maggie | FR | Mexico High School | NT | Unverified: |