| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | BAKER, Lucy | SO | Christian Fellowship School | 1:12.99 | Verified |
| 4 | SATTERFIELD, Moravia | SO | Southern Boone County High Schoo | 56.05 | Verified |
| 5 | ELIAS, Emily | SO | Fulton High School | 57.00 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WEAVER, Olivia | SO | Fulton High School | 55.39 | Verified |
| 2 | SEIBERT, Emmaleigh | FR | Mexico High School | 52.68 | Verified |
| 3 | SCHOENEBERG, Avery | SO | Blair Oaks High School | 50.49 | Verified |
| 4 | HANNAM, Zoey | SO | Moberly High School | 47.82 | Verified |
| 5 | MORRIS, Effie | FR | Boonville High School | 49.10 | Verified |
| 6 | SAMUELS, Eleanor | JR | Southern Boone County High Schoo | 51.77 | Verified |
| 7 | GROSS, Ariyonna | FR | Moberly High School | 54.80 | Verified |
| 8 | FESSLER, Charlotte | FR | Blair Oaks High School | 55.48 | Unverified: |