| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FOOKS, Simon | FR | Blair Oaks High School | 1:06.27 | Verified |
| 2 | HUTCHINS, Jeremiah | SR | Fulton High School | 1:02.13 | Verified |
| 3 | STRAATMANN, Riley | FR | Southern Boone County High Schoo | 59.63 | Verified |
| 4 | LOZANO, Adrian | SO | Fulton High School | 56.78 | Verified |
| 5 | SALINAS, Victor | SR | Missouri Military Academy | 57.41 | Verified |
| 6 | CAUDLE, Kaiden | FR | Blair Oaks High School | 1:01.42 | Verified |
| 7 | WALLACE, Matthew | SR | Christian Fellowship School | 1:04.73 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FRAME, Davontae | SO | Mexico High School | 56.52 | Verified |
| 2 | CHARLIE) FISHER, Charles | FR | Mexico High School | 56.13 | Verified |
| 3 | STOCK, Eli | JR | Boonville High School | 55.44 | Verified |
| 4 | GELINA, Brett | SR | Moberly High School | 50.21 | Verified |
| 5 | BRACKMAN, Ross | JR | Boonville High School | 52.31 | Verified |
| 6 | TRITTLER, Lance | SO | Southern Boone County High Schoo | 55.75 | Verified |
| 7 | POWELL, Bryson | SR | Missouri Military Academy | 56.46 | Verified |
| 8 | HORN, Dane | JR | Christian Fellowship School | 56.68 | Verified |