| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | ZOELLER, Sophia | SO | Boonville High School | NT | |
| 3 | TREMAIN, Ella | SO | Moberly High School | 1:11.43 | Verified |
| 4 | RAINES, Ella | JR | Mexico High School | 1:08.32 | Verified |
| 5 | CONNOR, Arianna | SO | Fulton High School | 1:11.41 | Verified |
| 6 | PHILLIPPE, Allysun | FR | Fulton High School | 1:11.56 | Verified |
| 7 | PAYNE, Mariah | SO | Boonville High School | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROSS, Chloe | SR | Moberly High School | 1:07.60 | Verified |
| 2 | YODER, Taylor | SR | Blair Oaks High School | 1:05.34 | Verified |
| 4 | HESTON, Grace | FR | Christian Fellowship School | 1:01.45 | Verified |
| 5 | GERMANN, Taylor | SO | Southern Boone County High Schoo | 1:02.17 | Verified |
| 7 | TERRY, Alexandra | FR | Mexico High School | 1:06.41 | Verified |
| 8 | FORCK, Chelsi | JR | Blair Oaks High School | 1:07.85 | Verified |