| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Blair Oaks High School | A | Christian | Kesel | Stockman | Roling | 48.72 | Verified |
| 2 | Fulton High School | A | 47.19 | Verified | ||||
| 3 | Southern Boone County High Schoo | A | Amelunke | Dapkus | Jennings | Marc | 44.72 | Verified |
| 4 | Mexico High School | A | Grimes | Grubb | Shivers | Shelton | 43.73 | Verified |
| 5 | Moberly High School | A | Alexander | Borges | Rasico | Flood | 44.48 | Verified |
| 6 | Missouri Military Academy | A | Erskine | Salinas | Burgett | Carter | 46.96 | Verified |
| 7 | Boonville High School | A | Norbury | Leonard | Stock | Martinez-Kurtti | 47.26 | Verified |