| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Boonville High School | A | Zoeller | Payne | Bledsoe | Thompson | 1:00.00 | Unverified: |
| 3 | Blair Oaks High School | A | Caviness | Willson | Schoeneberg | Vaughan | 53.64 | Verified |
| 4 | Moberly High School | A | Dunwoody | Fanning | Hannam | Gross | 51.99 | Verified |
| 5 | Fulton High School | A | Fleetwood | Richmond | Cooper | Begemann | 53.07 | Verified |
| 6 | Southern Boone County High Schoo | A | Chaney | Johnson | Patton | Patton | 55.01 | Verified |