| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HARTLEY, Ellie | FR | Elsberry High School | 5:55.03 | Verified |
| 2 | ELLIS, Matera | SR | Mark Twain High School | 6:01.00 | Verified |
| 3 | HAYS, Ella | JR | Monroe City High School | 6:10.65 | Verified |
| 4 | DEJAYNES, Michelle | SO | Pittsfield | 6:11.35 | Verified |
| 5 | JOHNSON, Avery | SO | Monroe City High School | 6:15.41 | Verified |
| 6 | BIBB, Dianna | SO | Bowling Green High School | 6:32.51 | Verified |
| 7 | FORMAN, Brooklyn | JR | Van-Far High School | 6:36.30 | Verified |
| 8 | LILLEY, Addison | FR | Bowling Green High School | 6:42.13 | Verified |
| 9 | TOELKE, Katelyn | SO | Pittsfield | 6:49.65 | Verified |