| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Clopton High School | A | Burt | West | Jolly | Harvey | 1:47.83 | Verified |
| 3 | Wellsville-Middletown High Schoo | A | Alsop | Henderson | Mandrell | Moore | 1:43.72 | Verified |
| 4 | Van-Far High School | A | Baugh | Gaston | Gibson | Selsor | 1:44.19 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mark Twain High School | A | Tobin | Zumwalt | Bennett | Bergthold | 1:39.09 | Verified |
| 2 | Pittsfield | A | Bonds | Jackson | Graham | Poor | 1:38.84 | Verified |
| 3 | Louisiana High School | A | Dzierwa | Luck | Harrison | Greene | 1:36.11 | Verified |
| 4 | Monroe City High School | A | Jones | Karr | Lehenbauer | Woolen | 1:38.18 | Verified |
| 5 | Bowling Green High School | A | Grote | Dunn | Lewis | Niemeyer | 1:39.00 | Verified |