| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Wellsville-Middletown High Schoo | A | Boeckelman | Boeckelman | Scott | Stroup | 2:10.88 | Verified |
| 3 | Van-Far High School | A | Brooksher | Gay | Engh-Hoffman | Shelton | 2:08.90 | Verified |
| 4 | Clopton High School | A | Palmer | Myers | Simmons | Bankhead | 2:10.61 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Monroe City High School | A | Miller | Lehenbauer | Clark | Hays | 2:04.13 | Verified |
| 2 | Mark Twain High School | A | Boleach | Hudelson | Dotson | Ross | 1:59.53 | Verified |
| 3 | Louisiana High School | A | Campbell | Ries | Chatman | Pedersen | 1:55.50 | Verified |
| 4 | Pittsfield | A | Waters | Wyatt | Preist | Patterson | 1:57.00 | Verified |
| 5 | Bowling Green High School | A | Dean | Johnson | Charlton | Gamm | 2:02.19 | Verified |