| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Monroe City High School | A | Woolen | Jones | Karr | Wheeler | 3:45.32 | Verified |
| 2 | Van-Far High School | A | Baugh | Hayden | Selsor | Hartley | 4:24.11 | Verified |
| 3 | Pittsfield | A | Oitker | Olson | Veile | Petty | 3:51.18 | Verified |
| 4 | Wellsville-Middletown High Schoo | A | Alsop | Bickell | Marshall | Moore | 4:00.16 | Verified |
| 5 | Louisiana High School | A | Martin | Lumley | Harrison | Dzierwa | 3:43.57 | Verified |
| 6 | Mark Twain High School | A | Preston | Miller | Northcutt | McLeod | 3:37.67 | Verified |