| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clopton High School | A | Palmer | Simmons | Myers | Talbert | NT | Verified |
| 2 | Mark Twain High School | A | Hudelson | Norton | Dotson | Miller | 4:41.36 | Verified |
| 3 | Pittsfield | A | Waters | Ten Eyck | Dejaynes | Wyatt | 4:28.57 | Verified |
| 4 | Louisiana High School | A | Campbell | Ries | Bowser | Pedersen | 4:40.17 | Verified |
| 5 | Monroe City High School | A | Clark | Foss | Hays | Johnson | 4:50.54 | Verified |